Overcoming the acoustic diffraction limit in photoacoustic imaging by localization of flowing absorbers

The resolution of photoacoustic imaging deep inside scattering media is limited by the acoustic diffraction limit. In this work, taking inspiration from super-resolution imaging techniques developed to beat the optical diffraction limit, we demonstrate that the localization of individual optical absorbers can provide super-resolution photoacoustic imaging well beyond the acoustic diffraction limit. As a proof-of-principle experiment, photoacoustic cross-sectional images of microfluidic channels were obtained with a 15 MHz linear CMUT array while absorbing beads were flown through the channels. The localization of individual absorbers allowed to obtain super-resolved cross-sectional image of the channels, by reconstructing both the channel width and position with an accuracy better than $\lambda/10$. Given the discrete nature of endogenous absorbers such as red blood cells, or that of exogenous particular contrast agents, localization is a promising approach to push the current resolution limits of photoacoustic imaging

Super-resolution photoacoustic imaging via flow induced absorption fluctuations

In deep tissue photoacoustic imaging the spatial resolution is inherently limited by the acoustic wavelength. We present an approach for surpassing the acoustic diffraction limit by exploiting temporal fluctuations in the sample absorption distribution, such as those induced by flowing particles. In addition to enhanced resolution, our approach inherently provides background reduction, and can be implemented with any conventional photoacoustic imaging system. The considerable resolution increase is made possible by adapting notions from super-resolution optical fluctuations imaging (SOFI) developed for blinking fluorescent molecules, to flowing acoustic emitters. By generalizing SOFI mathematical analysis to complex valued signals, we demonstrate super-resolved photoacoustic images that are free from oscillations caused by band-limited detection. The presented technique holds potential for contrast-agent free micro-vessels imaging, as red blood cells provide a strong endogenous source of naturally fluctuating absorption

Compensating for visibility artefacts in photoacoustic imaging with a deep learning approach providing prediction uncertainties

Conventional photoacoustic imaging may suffer from the limited view and bandwidth of ultrasound transducers. A deep learning approach is proposed to handle these problems and is demonstrated both in simulations and in experiments on a multi-scale model of leaf skeleton. We employed an experimental approach to build the training and the test sets using photographs of the samples as ground truth images. Reconstructions produced by the neural network show a greatly improved image quality as compared to conventional approaches. In addition, this work aimed at quantifying the reliability of the neural network predictions. To achieve this, the dropout Monte-Carlo procedure is applied to estimate a pixel-wise degree of confidence on each predicted picture. Last, we address the possibility to use transfer learning with simulated data in order to drastically limit the size of the experimental dataset.Comment: main text 10 pages + Supplementary materials 6 page

Photoacoustic fluctuation imaging: theory and application to blood flow imaging

Photoacoustic fluctuation imaging, which exploits randomness in photoacoustic generation, provides enhanced images in terms of resolution and visibility, as compared to conventional photoacoustic images. While a few experimental demonstrations of photoacoustic fluctuation imaging have been reported, it has to date not been described theoretically. In the first part of this work, we propose a theory relevant to fluctuations induced either by random illumination patterns or by random distributions of absorbing particles. The theoretical predictions are validated by Monte Carlo finite-difference time-domain simulations of photoacoustic generation in random particle media. We provide a physical insight into why visibility artefacts are absent from second-order fluctuation images. In the second part, we demonstrate experimentally that harnessing randomness induced by the flow of red blood cells produce photoacoustic fluctuation images free of visibility artefacts. As a first proof of concept, we obtain two-dimensional images of blood vessel phantoms. Photoacoustic fluctuation imaging is finally applied in vivo to obtain 3D images of the vascularization in a chicken embryo

Impact of Delayed Acknowledgment on TCP performance over LEO satellite constellations

This paper aims at quantifying the impact of a default TCP option, known as Delayed Acknowledgment (DelAck), in the context of LEO satellite constellations. Satellite transmissions can suffer from high channel impairments, especially on the link between a satellite and a ground gateway. To cope with these errors, physical and link layer reliability schemes have been introduced, at the price of an increase of the end-to-end delay seen by the transport layer (e.g. TCP). Although DelAck is used to decrease the feedback path load and for overall system performance, the use of this option conjointly with satellite link layer recovery schemes might increase the delay and might be counterproductive. To assess the impact of this option, we drive simulation measurements with two well-deployed TCP variants. The results show that the performance gain depends on the variant used and that this option should be carefully set or disabled as a function of the network characteristics. DelAck has a negative impact on TCP variants which are more aggressive such as TCP Hybla, and should be disabled for these versions. However, it shows benefits for TCP variants less aggressive such as NewReno

Super-resolution photoacoustic and ultrasound imaging with sparse arrays

It has previously been demonstrated that model-based reconstruction methods relying on a priori knowledge of the imaging point spread function (PSF) coupled to sparsity priors on the object to image can provide super-resolution in photoacoustic (PA) or in ultrasound (US) imaging. Here, we experimentally show that such reconstruction also leads to super-resolution in both PA and US imaging with arrays having much less elements than used conventionally (sparse arrays). As a proof of concept, we obtained super-resolution PA and US cross-sectional images of microfluidic channels with only 8 elements of a 128-elements linear array using a reconstruction approach based on a linear propagation forward model and assuming sparsity of the imaged structure. Although the microchannels appear indistinguishable in the conventional delay-and-sum images obtained with all the 128 transducer elements, the applied sparsity-constrained model-based reconstruction provides super-resolution with down to only 8 elements. We also report simulation results showing that the minimal number of transducer elements required to obtain a correct reconstruction is fundamentally limited by the signal-to-noise ratio. The proposed method can be straigthforwardly applied to any transducer geometry, including 2D sparse arrays for 3D super-resolution PA and US imaging

Making H-ARQ suitable for a mobile TCP receiver over LEO satellite constellations

This paper investigates strategies to carry out delay tolerant services over LEO satellite constellations for mobile receiver. In this context, LEO constellations are characterized by important delay variations where propagation impairments are mostly localized on the Land Mobile Satellite (LMS) channel (i.e. on the last hop). To cope with this issue, distinct reliability schemes can be introduced at the physical or link layers. Although their capacity to cope with transmission errors has been demonstrated, these recovery schemes may induce a high jitter that could severely damage TCP's internal timers and reliability schemes. As a matter of fact, transport and link layers’ reliability schemes exhibit a clear discrepancy. Following temporal traces representing the delay between a mobile terminal and the last hop satellite from a LEO constellation, we assess how HARQ mechanisms impact on the RTO based retransmission and the duplicate acknowledgments of TCP. Based on ns-2 simulations, we propose a layer-2 buffer that let both link and transport layers to conjointly perform. Our evaluations show an end-to-end data rate increase and more generally illustrate the benefit of re-ordering packets at the link layer when link-layer erasure coding recovery mechanisms are used conjointly with TCP

Shear wave pulse compression for dynamic elastography using phase-sensitive optical coherence tomography

Assessing the biomechanical properties of soft tissue provides clinically valuable information to supplement conventional structural imaging. In the previous studies, we introduced a dynamic elastography technique based on phase-sensitive optical coherence tomography (PhS-OCT) to characterize submillimetric structures such as skin layers or ocular tissues. Here, we propose to implement a pulse compression technique for shear wave elastography. We performed shear wave pulse compression in tissue-mimicking phantoms. Using a mechanical actuator to generate broadband frequency-modulated vibrations (1 to 5 kHz), induced displacements were detected at an equivalent frame rate of 47 kHz using a PhS-OCT. The recorded signal was digitally compressed to a broadband pulse. Stiffness maps were then reconstructed from spatially localized estimates of the local shear wave speed. We demonstrate that a simple pulse compression scheme can increase shear wave detection signal-to-noise ratio ([Formula: see text] gain) and reduce artifacts in reconstructing stiffness maps of heterogeneous media

Scheduling flows over LEO constellations on LMS channels

Satellite systems typically use physical and link layer reliability schemes to compensate the significant channel impairments, especially for the link between a satellite and a mobile end-user. These schemes have been introduced at the price of an increase in the end-to-end delay, high jitter or out-of-order packets. This is show to have a negative impact both on multimedia and best-effort traffic, decreasing the Quality of Experience (QoE) of users. In this paper, we propose to solve this issue by scheduling data transmission as a function of the channel condition. We first investigate existing scheduling mechanisms and analyze their performance for two kinds of traffic: VoIP and best-effort. In the case of VoIP traffic, the objective is to lower both latency and jitter, which are the most important metrics to achieve a consistent VoIP service. We select the best candidate among several schedulers and propose a novel algorithm specifically designed to carry VoIP over LEO constellations. We then investigate the performance of the scheduling policies on Internet-browsing traffic carried by TCP, where the goal is now the maximize the users’ goodput, and select the best candidate in this case

Controlled Delay Scheduler for VoIP over LEO constellations on LMS channels

Satellite transmissions can suffer from high channel impairments, especially on the link between a satellite and a mobile end-user. To cope with these errors, physical and link layer reliability schemes have been introduced at the price of an end-to-end delay increase resulting in high jitter. Unfortunately, both the delay and the jitter negatively impacts on multimedia traffic. As a matter of fact, not taking into account the channel state greatly decreases the Quality of Experience (QoE) of VoIP users. In this paper, we propose to solve this issue by scheduling data transmission as a function of the channel condition. We first investigate existing scheduling mechanisms and analyze their performance for VoIP traffic with the objective to lower both latency and jitter, which are the most important metrics to achieve a consistent VoIP service. We select the best candidate among several schedulers and propose a novel algorithm specifically designed to carry VoIP over LEO constellations. Our simulations show that in some scenarios, we double the QoE of VoIP users